Illumina-based identification of arbuscular mycorrhizal fungi from Karachay-Cherkessia soils for development of bio-fertilizers

The aim of the study was to investigate species diversity AM fungi in different parts North Caucasus, biodiversity hotspot, center world’s biological diversity. Samples were taken from 5 locations (stationary trial plots, STPs) ecosystems and at various altitudes. Identification performed using sequencing for ITS1 ITS2 regions, amplified with universal primers, Illumina MiSeq employed. 19 genera found on all STPs. work did not reveal a correlation between altitude composition fungi. At same time, it should be assumed that could type ecosystem, which done future. shows is necessary use an analysis both ITS since data obtained each region differ complement other. Analysis revealed 1.3 times more virtual taxa than ITS1, while number OTUs identified per similar regions. highest STP #3 (with meadow flora). Only 4 ( Rhizophagus irregularis, R. intraradices, Paraglomus laccatum, Claroideoglomus claroideum ) five analyzed We unexpectedly such high among fungi, no genus, sequences belonged laccatum , whereas least 9 are distinguished genus by morphology. Further research will allow us identify new strains efficiency may higher already studied ones. In future this make possible create effective microbial biofertilizers agriculture.